BS3 Crosslinker (CAS: 82436-77-9)
BS3 Crosslinker, also known as bis(sulfosuccinimidyl)suberate, is a water-soluble, non-cleavable crosslinker designed for bioconjugation and protein interaction studies. It contains two sulfo-NHS ester groups that specifically react with primary amines on lysine residues or protein N-termini, forming stable amide bonds. Therefore, it is highly effective for creating permanent protein-protein linkages in aqueous environments.
Key Features:
- Water-Soluble: Easily dissolves in water, eliminating the need for organic solvents.
- Non-Cleavable: Forms permanent crosslinks, ensuring long-term stability.
- Membrane-Impenetrable: Since its charged structure prevents it from crossing cell membranes, BS3 is ideal for labeling cell surface proteins.
BS3 Crosslinker Applications:
- Protein-Protein Conjugation: Links proteins by forming stable amide bonds between primary amines.
- Receptor-Ligand Studies: Helps study cell surface receptor interactions.
- Immunoprecipitation: Crosslinks proteins on cell surfaces before cell lysis, preserving protein complexes for analysis.
Handling and Storage:
- Molecular Formula: C₁₆H₁₈N₂Na₂O₁₄S₂
- Molecular Weight: 572.43 g/mol
- Spacer Arm Length: 11.4 Å (8 atoms)
- Storage: Store at 2-8°C, protected from moisture.
BS3 Crosslinker is commonly used in biochemical and molecular biology research for studying protein interactions and creating stable protein complexes in aqueous environments.
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