Boc-Aminooxyacetamide-PEG3-propargyl
Boc-Aminooxyacetamide-PEG3-propargyl is a heterobifunctional PEG linker featuring a Boc-protected aminooxy group and a terminal alkyne (propargyl) group. Additionally, the PEG3 spacer enhances water solubility and provides molecular flexibility. As a result, this reagent is ideal for orthogonal bioconjugation, enabling both oxime ligation with aldehyde/ketone groups and copper-catalyzed click chemistry with azides.
Key Features and Benefits:
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Boc-Protected Aminooxy Group: Offers selective oxime bond formation after deprotection; ideal for conjugating with aldehyde- or ketone-modified biomolecules.
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Propargyl Alkyne Functionality: Compatible with copper-catalyzed azide-alkyne cycloaddition (CuAAC), allowing robust click chemistry labeling.
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PEG3 Spacer: Improves aqueous solubility, reduces aggregation, and allows spatial separation of reactive groups.
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Orthogonal Reactivity: Enables dual labeling or modular conjugation workflows with high specificity.
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High Purity: Supplied at >95% purity to support sensitive bioconjugation and bioassay development.
Applications:
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Dual-Functional Bioconjugation: Attach one end via click chemistry and the other via oxime ligation for branched or multifunctional constructs.
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Protein and Glycoprotein Labeling: Conjugate to oxidized glycan or N-terminal aldehydes on proteins.
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Diagnostic Probe Design: Develop bifunctional imaging agents or sensors with precise control.
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Nanoparticle Functionalization: Introduce bioorthogonal handles on surfaces for downstream modification.
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Drug Delivery Research: Build modular, responsive delivery systems with cleavable or triggerable components.
Storage and Handling:
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Storage: Store at -20°C, away from light and moisture.
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Handling: Deprotect the Boc group using 50% TFA in DCM or equivalent. Use dry solvents such as DMSO or DMF to preserve reactivity.
Boc-Aminooxyacetamide-PEG3-propargyl provides a powerful platform for orthogonal conjugation strategies in chemical biology, diagnostic assay development, and therapeutic design. Specifically, Its dual-reactive architecture allows precise and modular assembly of complex bioconjugates.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.