Biotin-Hydrazide
This product is a high-purity biotin derivative featuring a hydrazide group for selective coupling with aldehydes and ketones. The biotin moiety enables strong, specific binding to streptavidin or avidin, while the hydrazide allows covalent labeling of oxidized glycans, aldehyde-tagged biomolecules, or other carbonyl-containing targets. This makes it especially useful in affinity purification, labeling, and detection workflows.
Key Features and Benefits
-
Dual Functionality: Biotin for affinity binding, hydrazide for carbonyl conjugation.
-
High Affinity: Strong interaction with streptavidin/avidin systems.
-
Specific Reactivity: Targets aldehydes and ketones for stable hydrazone bonds.
-
High Purity: Suitable for sensitive biochemical applications.
Applications
-
Affinity purification of carbonyl-containing biomolecules.
-
Glycoprotein labeling after periodate oxidation.
-
Biotinylation of aldehyde-tagged proteins and peptides.
-
Detection in ELISA, Western blotting, and other biotin–streptavidin assays.
Storage and Handling
-
Storage: Store at –20 °C in a dry, dark place.
-
Handling: Protect from moisture and light; use under inert atmosphere if possible.
This product is a versatile reagent, especially ideal for labeling, purification, and detection in biochemical assays.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
