Biotin-bisamido-SS-NHS
This product is a cleavable biotinylation reagent featuring an NHS ester for efficient amine coupling, a biotin moiety for strong avidin/streptavidin binding, and a disulfide bridge for reductive cleavage. Its design offers high solubility, stable conjugation, and controlled release, making it especially valuable in labeling, detection, and enrichment applications.
Key Features and Benefits:
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Biotin Moiety: Ensures strong, specific binding to avidin and streptavidin.
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NHS Ester: Couples efficiently with amines to form stable amide bonds.
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Disulfide Linker: Provides reductive cleavability for reversible labeling.
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Bisamido Structure: Enhances stability and compatibility with biomolecules.
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High Purity: Ensures reproducibility in demanding assays.
Applications:
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Protein Labeling: Targets primary amines for biotinylation.
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Detection and Enrichment: Supports streptavidin-based pull-down and capture assays.
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Reversible Conjugation: Enables controlled release under reducing conditions.
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Probe Development: Builds cleavable biotin probes for diagnostics and imaging.
Storage and Handling:
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Storage: Store at –20 °C in a sealed, light- and moisture-protected container.
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Handling: Use anhydrous solvents (e.g., DMF, DMSO); avoid premature hydrolysis.
This product provides stable, amine-reactive biotinylation with reductive cleavability, specifically offering versatility for labeling, enrichment, and controlled bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
