BDP TMR Carboxylic Acid (CAS: 287384-28-5)
BDP TMR Carboxylic Acid is a highly fluorescent dye from the BODIPY family, recognized for its exceptional brightness, photostability, and chemical versatility. Since its carboxylic acid group enables easy attachment to biomolecules like proteins and peptides, it is widely used in fluorescence-based applications such as microscopy and fluorescence polarization assays. Its strong fluorescence and stable chemical structure make it an essential tool in advanced biological research.
Key Features:
Fluorescent Properties:
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- Excitation Maximum: ~542 nm
- Emission Maximum: ~574 nm
- High Fluorescence Yield: Ensures bright, stable, and long-lasting signals, making it suitable for demanding applications
Reactive Group:
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- Its carboxylic acid group easily couples with primary amines using activators like EDC or HATU, forming strong amide bonds for biomolecule labeling. Consequently, this allows efficient and precise bioconjugation.
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Solubility:
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- Since it is soluble in alcohols, DMSO, and DMF, it supports a variety of experimental setups and chemical reactions.
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Applications:
- Microscopy:
- Due to its bright and stable fluorescence, BDP TMR Carboxylic Acid delivers high-resolution imaging, making it ideal for cellular and molecular visualization.
- Fluorescence Polarization Assays:
- Its strong fluorescence enables accurate studies of molecular interactions, supporting binding and activity assays.
Handling and Storage:
- Molecular Formula: C₂₁H₂₁BF₂N₂O₃
- Molecular Weight: 398.22 g/mol
- Storage: Store at -20°C in a dark, dry place to maintain long-term stability and avoid degradation.
Overall, BDP TMR Carboxylic Acid is a versatile and powerful fluorescent dye widely used in biological research. Its strong photostability, excellent reactivity, and chemical compatibility ensure consistent performance in labeling, detection, and imaging applications. Therefore, it remains an essential tool for researchers in molecular biology and advanced fluorescence-based studies.
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