BCN-PEG3-NHS ester (CAS: 2101206-94-2) is a versatile chemical used for bioconjugation, drug delivery, and material science applications. It has three key components: a BCN (bicyclononyne) group, a PEG3 spacer, and an NHS ester group, each serving a specific function.
Key Features:
- BCN Group: This part of the molecule allows for copper-free click chemistry by reacting efficiently with azides. The BCN-azide reaction is fast, non-toxic, and produces stable triazole linkages, making it perfect for sensitive biological systems, including live-cell labeling and in vivo studies.
- PEG3 Spacer: The PEG3 is a biocompatible polyethylene glycol molecule with two repeating units. It improves water solubility, bioavailability, and reduces immunogenicity, making it ideal for conjugation in biological applications. The spacer also adds flexibility, ensuring easy conjugation with other molecules.
- NHS Ester Group: The NHS ester reacts with primary amines on proteins, peptides, or other biomolecules, forming strong and stable amide bonds. This is essential for effective bioconjugation reactions.
Applications:
- Protein and Peptide Labeling: The NHS ester allows BCN-PEG3-NHS ester to efficiently bond with proteins, peptides, and other biomolecules, forming stable conjugates for a wide range of research and clinical applications.
- Copper-Free Click Chemistry: The BCN group makes it ideal for copper-free SPAAC (strain-promoted azide-alkyne cycloaddition) reactions. This is especially useful in bio-orthogonal chemistry, where copper needs to be avoided for live-cell or in vivo labeling.
- Drug Delivery & Material Science: The PEG3 spacer helps enhance the solubility and bioavailability of the conjugated molecules, reducing aggregation and improving the performance of drug delivery systems. In material science, this product is widely used for surface functionalization and nanoparticle modifications.
Why Choose BCN-PEG3-NHS Ester?
- Copper-Free and Biocompatible: The BCN group allows fast, safe, and non-toxic reactions with azides, making it perfect for sensitive biological systems.
- Efficient Conjugation: With both NHS ester and BCN functionalities, this compound is highly flexible and can be used for various conjugation strategies.
- Improved Solubility: The PEG3 spacer increases water solubility and bioavailability, which is critical for drug delivery and biological studies.
Storage and Handling:
- Storage: Keep BCN-PEG3-NHS ester stored at -20°C in a dry environment to maintain its stability.
- Handling: Follow proper lab safety procedures when handling, especially since the NHS ester is sensitive to moisture.
This product is widely used in molecular biology, pharmaceutical development, and biotechnology for its versatility in bioconjugation, drug delivery, and surface modifications. It offers efficient, copper-free reactions that are safe for biological applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
