Azido-PEG3-CH2CO2-NHS
Azido-PEG3-CH2CO2-NHS is a high-purity heterobifunctional PEG linker with a terminal azide for copper-catalyzed or strain-promoted click chemistry and an NHS ester-activated carboxylic acid for rapid amine coupling. The PEG3 spacer enhances solubility, flexibility, and reduces steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. This design is ideal for orthogonal conjugation strategies requiring sequential amine and azide modifications.
Key Features and Benefits
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Azide Group: Reacts via CuAAC (copper-catalyzed azide–alkyne cycloaddition) or SPAAC (strain-promoted cycloaddition) with alkynes for bioorthogonal labeling.
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NHS Ester: Rapidly couples with primary amines under mild conditions to form stable amide bonds.
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PEG3 Spacer: Improves water solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Enables orthogonal and sequential modification strategies.
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High Purity: Supplied at >95% purity for reliable and reproducible performance.
Applications
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Stepwise Bioconjugation: Sequentially couple amines and alkynes in orthogonal fashion.
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Protein and Peptide Labeling: Introduce azide handles while attaching other functional moieties.
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Nanoparticle and Surface Modification: Add clickable azide groups to amine-functionalized surfaces.
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Drug Delivery Systems: Build multifunctional linkers for targeted payload release.
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Imaging and Diagnostic Probes: Prepare dual-labeled probes with orthogonal chemistries.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged moisture exposure to maintain NHS ester reactivity.
Azido-PEG3-CH2CO2-NHS combines an azide for click chemistry with an NHS ester for efficient amine coupling in a hydrophilic PEG3 framework, offering versatility for orthogonal bioconjugation specifically in therapeutics, diagnostics, and materials science.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
