APDye™ 488 NHS Ester is a bright, green-emitting dye equivalent to Alexa Fluor® 488 NHS Ester, designed for labeling proteins, antibodies, amine-modified oligonucleotides, and other amine-containing biomolecules. Its NHS ester group reacts quickly with primary amines, such as lysine residues, forming stable amide bonds for consistent labeling. This dye is widely used in fluorescence microscopy, flow cytometry, and immunofluorescence due to its high brightness and stability.
Key Features:
- Vivid Green Fluorescence: Excites at 493 nm and emits at 518 nm, delivering clear and strong green signals for imaging.
- Rapid Amine Reactivity: Efficiently labels primary amines on proteins and peptides, ensuring reliable and stable conjugation.
- Alexa Fluor® 488 Equivalent: Matches spectral properties, making it easy to integrate into existing protocols without adjustments.
Applications:
- Protein and Antibody Labeling: Tags proteins and antibodies through primary amines, enhancing detection in various fluorescence assays.
- Flow Cytometry: Provides bright, specific signals for cell sorting and precise analysis.
- Immunofluorescence: Offers high-contrast, stable fluorescence for clear visualization in cellular studies.
Benefits:
- Fast Conjugation: The NHS ester reacts swiftly with amines, securing strong, durable bonds.
- High Sensitivity: Delivers strong fluorescence, ideal for detecting low-abundance biomolecules.
- Versatile Use: Fits a broad range of applications in research, diagnostics, and imaging.
Storage and Handling:
- Store at -20°C, away from light and moisture to preserve stability.
- Handle using standard precautions for fluorescent dyes.
APDye™ 488 NHS Ester stands out for its bright, stable performance in biological research, diagnostics, and advanced imaging.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.