AF594 NHS Ester, 5-Isomer
Description: AF594 NHS Ester, 5-Isomer, is a bright, water-soluble red-fluorescent dye that remains stable across a pH range of 4 to 10, making it suitable for various biological conditions. It is commonly applied in flow cytometry and fluorescent microscopy for accurate, high-contrast imaging.
Key Features:
- Stable Fluorescence: The dye is pH-resistant (4–10) and provides vivid, stable red fluorescence, suitable for long-lasting imaging.
- High Labeling Efficiency: Optimized for labeling proteins, peptides, antibodies, and -NH₂-containing molecules like amino-modified oligonucleotides.
- Optimal Conjugation Conditions: The NHS ester reacts efficiently with primary amines, forming stable amide bonds at pH 7–9 for the best conjugation results.
Applications:
- Protein Labeling: AF594 allows a high molar dye-to-protein ratio, producing conjugates with a high degree of labeling (DOL). These conjugates exhibit minimal fluorescence quenching, resulting in brighter fluorescence and an improved detection limit.
- Microscopy & Flow Cytometry: The dye’s stability and brightness make it ideal for cellular imaging and flow cytometry, enhancing the sensitivity and accuracy of detection.
- Bioconjugation: Used for robust and stable tagging of biomolecules, AF594 is valuable in biochemical assays requiring consistent fluorescence output.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
