AF647 NHS Ester (Alexa Fluor 647 NHS Ester equivalent) is a bright, far-red fluorescent dye designed for labeling proteins, peptides, antibodies, and amine-modified oligonucleotides. It excels in imaging and flow cytometry, providing stable, vivid fluorescence ideal for visualization.
Spectral Properties:
- Excitation: 655 nm
- Emission: 680 nm
- Molar Extinction Coefficient: 191,800 L⋅mol⁻¹⋅cm⁻¹
- Quantum Yield: 0.15
AF647 NHS Ester Key Features:
- Bright Far-Red Fluorescence: Optimized for 594 nm and 633 nm lasers, it offers intense and stable signals.
- High Solubility & pH Stability: Performs consistently from pH 4 to 10, making it versatile for various environments.
- Efficient Conjugation: The NHS ester binds directly to primary amines on biomolecules, enhancing labeling with minimal effort.
Applications:
- Bioconjugation: Label proteins, antibodies, and peptides easily, achieving high fluorescence and photostability.
- Imaging & Flow Cytometry: Produces clear, reliable signals for research and diagnostics.
- Better Performance: Outshines other red dyes in brightness and photostability, making it a top choice for detailed visualization tasks.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
