(2-pyridyldithio)-PEG4-NHS ester
(2-pyridyldithio)-PEG4-NHS ester is a high-purity heterobifunctional PEG linker featuring a 2-pyridyldisulfide group for thiol-reactive conjugation and an NHS ester for efficient amine coupling. The PEG4 spacer enhances solubility, flexibility, and reduces steric hindrance, enabling high-yield bioconjugation in both aqueous and organic systems. The orthogonal reactivity of the two functional groups allows stepwise conjugation, making this reagent ideal for precision linker design in protein modification, drug delivery, and advanced biomaterials.
Key Features and Benefits
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2-Pyridyldisulfide Group: Enables reversible or cleavable disulfide bond formation with thiols.
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NHS Ester: Rapidly reacts with primary amines to form stable amide linkages under mild conditions.
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PEG4 Spacer: Improves water solubility, flexibility, and accessibility of reactive sites.
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Heterobifunctional Design: Supports dual-step bioconjugation strategies with orthogonal chemistry.
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High Purity: >95% purity ensures reproducible and reliable performance.
Applications
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Protein and Peptide Modification: Introduce cleavable disulfide linkages while coupling amines.
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Antibody Conjugation: Attach payloads or labels via thiol and amine chemistries.
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Nanoparticle Functionalization: Add reversible linkers for targeted drug delivery.
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Drug Delivery Systems: Build linkers with controlled cleavage for intracellular release.
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Diagnostic Probe Development: Create cleavable imaging and sensing probes.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve NHS ester reactivity.
(2-pyridyldithio)-PEG4-NHS ester provides orthogonal thiol- and amine-reactive chemistries with excellent solubility, making it an especially versatile choice for constructing cleavable bioconjugates, precision linkers, and targeted delivery systems.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.