N-(2-Hydroxyethyl)maleimide CAS:1585-90-6 is a chemical compound commonly used in biochemistry and molecular biology. It is a maleimide derivative with a hydroxyethyl group attached to the nitrogen atom of the maleimide ring. N-(2-Hydroxyethyl)maleimide is a reactive compound that can selectively modify thiol groups on proteins, peptides, and other biomolecules, through a Michael addition reaction. This makes N-(2-Hydroxyethyl)maleimide a useful tool for studying protein structure and function, as well as for cross-linking and immobilizing biomolecules. N-(2-Hydroxyethyl)maleimide is also used in various applications such as protein purification, protein labeling, and in the development of biosensors. It is often preferred over other maleimide derivatives due to its water solubility and low toxicity.
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