Di-4-ANEPPS, potentiometric probe

Di-4-ANEPPS, CAS Number: 90134-00-2

Overview:
Di-4-ANEPPS is a fast-responding, voltage-sensitive dye from the ANEP family. It binds to cell membranes and fluoresces in response to electrical potential changes, making it ideal for tracking membrane potential in neurons, cardiac cells, and other excitable cells. Its quick optical response detects transient voltage shifts, causing a fluorescence change of 2-10% per 100 mV. It also enables ratiometric measurements by shifting its excitation spectrum with membrane potential changes.

Because Di-4-ANEPPS is rapidly internalized by cells, it is most effective for short-term studies. For longer retention, you can opt for Di-8-ANEPPS, which has higher hydrophobicity. In addition to its role in membrane potential tracking, Di-4-ANEPPS also acts as a plasma membrane and endocytosis marker.

Applications:

• Membrane potential studies: Perfect for monitoring real-time voltage changes in excitable cells.
• Neuroscience and cardiac research: Tracks fast electrical activity in neurons and cardiac cells.
• Ratiometric techniques: Ideal for measuring voltage changes via spectral shift.
• Membrane marker: Labels the plasma membrane and tracks endocytosis.

Excitation/Emission Maxima:

• In methanol: 496/705 nm
• In lipids/cell membranes: Blue-shifted compared to organic solvents.

Usage Guidelines:
To use Di-4-ANEPPS, add it to cell cultures with Pluronic® F-127 or through retrograde labeling. Start with a concentration of 5-10 μM, adjusting as needed based on experimental requirements.

Benefits:

• Responds quickly to voltage changes, ideal for real-time studies.
• High sensitivity, providing 2-10% fluorescence change per 100 mV.
• Suitable for voltage measurements and cell labeling in short-term studies.

Storage:
Store at -20°C, protected from light and moisture.

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