3-Cyanovinylcarbazole Phosphoramidite, CAS 1157899-72-3, is a specialized reagent for oligonucleotide synthesis. It contains a cyanovinyl group attached to a carbazole base, which allows photo-crosslinking with DNA or RNA upon UV exposure. Researchers use it in molecular biology, nucleic acid studies, and probe development.
Key Features
- Cyanovinyl Group: Enables photo-crosslinking with nucleic acids when exposed to UV light.
- Carbazole Base: Adds stability and boosts the functionality of the modified strand.
- Phosphoramidite Compatibility: Works seamlessly with standard DNA/RNA synthesis protocols for easy integration.
Applications
- Oligonucleotide Synthesis: Incorporates into DNA or RNA strands for targeted photo-crosslinking.
- Molecular Probes: Creates probes for detecting nucleic acids or studying molecular interactions.
- Nucleic Acid Research: Useful for exploring DNA/RNA structures and dynamics through crosslinking.
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