Fmoc-Glu(biotinyl-PEG)-OH: Advanced Peptide Synthesis and Applications

Introduction to Fmoc-Glu(biotinyl-PEG)-OH

Fmoc-Glu(biotinyl-PEG)-OH is a versatile and highly specialized peptide derivative used in cutting-edge biochemical research and pharmaceutical applications. Combining the Fmoc (Fluorenylmethyloxycarbonyl) protective group, glutamic acid (Glu), and a biotinylated polyethylene glycol (PEG) moiety, this compound is crucial for peptide synthesis and tagging.

Key Components

• Fmoc (Fluorenylmethyloxycarbonyl): A widely used protective group in peptide synthesis that facilitates the sequential addition of amino acids.
• Glu (Glutamic Acid): An amino acid involved in protein synthesis and modification.
• Biotinyl-PEG (Biotinylated Polyethylene Glycol): Enhances solubility, stability, and enables specific biotin-streptavidin interactions.

Applications of Fmoc-Glu(biotinyl-PEG)-OH

1. Peptide Synthesis

Fmoc-Glu(biotinyl-PEG)-OH is employed in solid-phase peptide synthesis (SPPS) to incorporate biotinylated PEG into peptides. This incorporation is essential for creating peptides with improved solubility and stability, which are crucial for various biochemical assays.

2. Biotin Labeling

The biotinyl group attached to PEG allows for easy and specific labeling of peptides. This labeling is pivotal in a range of applications, including:

• Affinity Purification: Biotin-streptavidin interactions facilitate the isolation and purification of peptides or proteins.
• Immunoassays: Enhances the detection sensitivity in assays like ELISA and Western blotting.

3. Cell Targeting and Imaging

Peptides tagged with biotinylated PEG can be used for targeted delivery and imaging studies. The biotin-streptavidin system allows for the precise targeting of cells or tissues, making it an invaluable tool in cell biology and molecular imaging.

4. Drug Development

In drug discovery, Fmoc-Glu(biotinyl-PEG)-OH plays a role in:

• Screening for Drug Interactions: Tagged peptides help identify and analyze drug-receptor interactions.
• Target Identification: Facilitates the identification of potential drug targets through affinity-based methods.

Advantages of Using Fmoc-Glu(biotinyl-PEG)-OH

• Enhanced Solubility and Stability: The PEG moiety improves the solubility and stability of peptides in aqueous solutions.
• Specific Labeling: The biotinyl group allows for highly specific interactions with streptavidin, enhancing the accuracy of assays.
• Versatility: Useful in a wide range of biochemical and pharmaceutical applications, from peptide synthesis to drug development.

Techniques Utilizing Fmoc-Glu(biotinyl-PEG)-OH

Mass Spectrometry (MS)

• Purpose: Accurate identification and quantification of peptides.
• Application: Analyzing peptide sequences and modifications.

Chromatography

• Purpose: Separation and purification of peptides.
• Application: High-performance liquid chromatography (HPLC) for purifying biotinylated peptides.

Immunoassays

• Purpose: Detection and quantification of specific peptides.
• Application: ELISA, Western blotting, and other antibody-based assays.

Cell-Based Assays

• Purpose: Investigating peptide interactions within live cells.
• Application: Tracking peptide delivery and cellular responses.

Ref: Ravi S, Krishnamurthy VR, Caves JM, Haller CA, Chaikof EL. Maleimide-thiol coupling of a bioactive peptide to an elastin-like protein polymer. Acta Biomater. 2012 Feb;8(2):627-35. 

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.