Cy3B NHS Ester – Superior Fluorescent Labeling for Enhanced Detection
Overview: Cy3B NHS Ester is an advanced version of Cy3. It is a bright, water-soluble, and pH-insensitive orange fluorescent dye that performs well across a pH range of 4 to 10. Ideal for labeling antibodies, peptides, and proteins, Cy3B conjugates can be excited with 532 nm or 555 nm laser lines and visualized using the TRITC (tetramethylrhodamine) filter set.
Why Choose Cy3B NHS Ester?
- Improved Fluorescence: Cy3B conjugates generate less background noise and provide superior fluorescence quantum yield and photostability compared to TAMRA and other common fluorochromes.
- Versatile Excitation: Compatible with 532 nm and 555 nm laser lines for flexible experimental setups.
Application Considerations:
- NHS Esters for Labeling: NHS esters (or succinimidyl esters) are widely used for labeling proteins, amine-modified oligonucleotides, and other amine-containing molecules. They react efficiently with primary amines (e.g., Lys residues).
- Target Abundance: Cy3B NHS Ester is best suited for medium to high-abundance targets due to its self-quenching properties. For detecting low-abundance targets, consider using APDye 555 NHS Ester (an Alexa Fluor® 555 analog), which allows for high molar ratio labeling with minimal self-quenching, resulting in brighter conjugates and enhanced sensitivity.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
