Universal Black 3 Quencher (UBQ-3), also known as Black Hole Quencher®-3 (BHQ®-3) equivalent, is a dark, far-red nonfluorescent acceptor dye that lacks native emission. It effectively quenches fluorescence, making it ideal for dual-labeled probes and qPCR applications.
Key Features:
- Fluorescence Quenching: UBQ-3 reduces background fluorescence in various assays.
- Broad Quenching Range: It operates between 620 and 730 nm, which makes it suitable for FRET applications with dyes like Cy5, Alexa Fluor® 647, and IRDye® 680.
- Structural Identity: Notably, UBQ-3 is structurally identical to BHQ®-3.
Key Applications:
- Fluorogenic Probes: Researchers use it as an acceptor in dual-labeled probes.
- qPCR Probes: It improves performance by minimizing background noise.
- FRET Studies: The dye serves as a reliable acceptor in studies of molecular interactions.
- Copper-Free Click Chemistry: UBQ-3 DBCO reacts with azides without needing copper catalysts, allowing for safe labeling of proteins, lipids, and peptides.
In summary, UBQ-3 enhances the sensitivity and reliability of fluorescence-based techniques while providing a safe option for chemical reactions.
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