Cyanine3 NHS Ester is a reactive dye used for labeling amino groups in proteins, peptides, and oligonucleotides/DNA. It serves as a reliable replacement for Cy3® and DyLight 549 NHS esters, forming stable amide bonds with primary amines. For delicate proteins, the water-soluble sulfo-Cyanine3 NHS ester is recommended as it avoids co-solvent use, preserving protein structure.
Key Features:
- Bright Emission: Provides bright orange-red fluorescence, optimized for Cy3 filter sets.
- Efficient Labeling: Rapidly labels primary amines in biomolecules.
- Versatile Replacement: Substitutes Cy3® and DyLight 549 NHS esters.
Spectral Properties:
- Excitation/Absorption Max: 555 nm
- Molar Extinction Coefficient (ε): 150,000 L⋅mol⁻¹⋅cm⁻¹
- Emission Max: 570 nm
- Fluorescence Quantum Yield: 0.31
- CF260: 0.04
- CF280: 0.09
Applications:
- Protein and Antibody Labeling: Ideal for imaging and diagnostics.
- DNA and Oligonucleotide Labeling: Enhances visualization in nucleic acid research.
- Flow Cytometry and Microscopy: Delivers bright, stable signals for detailed imaging.
Cyanine3 NHS Ester is a versatile and robust choice for precise fluorescent labeling across various bioanalytical and research applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
