What Is Ionizable Lipids?

Ionizable lipids are a category of lipid molecules that maintain a neutral charge at normal physiological pH but become positively charged when the pH is lowered, typically due to protonation. ALC-0315 is an example, featuring an amine site that can become protonated at lower pH levels. Ionizable lipids play a crucial role as a component in Ionizable lipid nanoparticles (LNPs), which are widely employed for the systemic delivery of RNA therapeutics. LNPs also consist of other lipid molecules such as helper lipids, cholesterol, PEG lipids, and more.

What are ionizable lipids examples?

Examples of ionizable lipids include ALC-0315, C12-200, cKK-E12, and DLin-MC3-DMA. These are lipid molecules that can become positively charged at lower pH levels, which makes them valuable components in the formulation of lipid nanoparticles (LNPs) used for the delivery of RNA therapeutics and other applications.

Ionizable Lipids Advantages

The pH-responsive nature of ionizable lipids is advantageous for in vivo mRNA delivery. This is because neutral lipids have fewer interactions with the negatively charged membranes of blood cells, leading to enhanced biocompatibility of lipid nanoparticles. When trapped within endosomes, where the pH is lower than in the surrounding extracellular environment, ionizable lipids undergo protonation, acquiring a positive charge. This can potentially induce membrane destabilization and aid in the escape of nanoparticles from the endosomes. In summary, ionizable lipids play a role in promoting endosomal escape and reducing the toxicity associated with lipid nanoparticles (LNPs) during mRNA delivery.

Application of Ionizable Lipids

Ionizable lipids have a variety of applications, including:
mRNA Vaccine Delivery: Ionizable lipids are used in lipid nanoparticles (LNPs) to encapsulate and deliver mRNA vaccines. They help protect the mRNA payload and promote its efficient delivery into cells.

Gene Therapy: Ionizable lipids are employed in gene therapy to deliver therapeutic genes into target cells. They facilitate the transport of genetic material across cell membranes.

Drug Delivery: Ionizable lipids are used in the formulation of lipid-based drug delivery systems. These systems can improve the solubility and bioavailability of poorly soluble drugs.

siRNA Delivery: Ionizable lipids are used to deliver small interfering RNA (siRNA) molecules to silence specific genes. This is particularly relevant in the development of RNA interference (RNAi) therapies.

Cancer Therapy: Ionizable lipids can be utilized in the delivery of anticancer drugs to tumor cells, enhancing drug efficacy while minimizing off-target effects.

Intracellular Delivery: They are used to facilitate the delivery of various types of therapeutic molecules, such as peptides, proteins, and small molecules, into cells for various medical applications.

Research Tools: Ionizable lipids are employed as research tools to study cellular processes, gene function, and the development of novel therapeutic strategies.

Axispharm – Professional Ionizable Lipids Supplier

Axispharm, a prominent global supplier of biochemicals, provides a diverse range of lipid molecules, including ionizable lipids, cationic lipids, helper lipids, and PEG lipids, to clients worldwide. Additionally, Axispharm offers rapid and customized synthesis services for innovative lipid molecules to support your advanced research endeavors. For inquiries, please contact