Intact Protein Analysis Services

At AxisPharm, we offer intact protein analysis to determine the total molecular weight of proteins without digestion or fragmentation. This powerful technique is ideal for characterizing complex biopharmaceuticals like monoclonal antibodies (mAbs). Using advanced mass spectrometry (MS), we deliver fast and accurate insights into protein structure, modifications, and heterogeneity.

What is Intact Mass Analysis?

Intact mass analysis measures the molecular weight of proteins in their full, undigested form. This approach is essential for studying complex proteins such as mAbs. It helps to:

  • Determine molecular weight
  • Characterize post-translational modifications (PTMs)
  • Identify protein heterogeneity
  • Detect sequence variations or degradation products

Intact mass analysis gives you a rapid and detailed look at your proteins, ensuring thorough characterization for research or development.

Why Use ESI-MS for Intact Protein Analysis?

  1. High Sensitivity and Accuracy:
    ESI-MS provides precise results, detecting even minor structural changes.

  2. Fast Detection:
    This method offers rapid protein analysis, saving you time.

  3. Combines with Chromatography:
    ESI-MS works well with chromatography to identify trace substances in complex systems.

  4. Ideal for Complex Systems:
    It effectively analyzes highly polar, difficult-to-volatilize, or thermally unstable compounds.

  5. Transition State Insights:
    ESI-MS reveals important information about protein composition and conformation, helping you understand protein behavior.

    Sample Requirements

    To ensure accurate results, we recommend the following sample guidelines:

        • Purity: >90%
        • Avoid Detergents: Avoid SDS, PEG, Triton, and other detergents.
        • Acceptable Buffers: Use ammonium acetate, bicarbonate, or formate.
        • Quantity: 10-40 µg
        • Concentration: For best results, ensure appropriate protein amounts (e.g., 25 pmol at 5 kDa, 100 pmol at 20 kDa).

    Technologies We Use

    AxisPharm uses several advanced methods for intact protein analysis:

    We analyze proteins up to 300 kDa, offering high precision for a variety of biopharmaceutical applications. Additionally, we provide SEC and FPLC protein purification services to ensure high-quality samples.

    Monoclonal Antibody (mAb) Analysis

    Monoclonal antibodies are vital biopharmaceuticals, and accurate analysis is critical at every stage of development. Intact mAb analysis quickly determines molecular weight and identifies heterogeneity, including PTMs, ADC linkages, and degradation products. Using Quadrupole Time-of-Flight (Q-TOF) LC/MS, we offer high-resolution analysis, giving you a detailed view of your mAb product.

    Benefits Over ELISA for mAb Analysis

    Unlike ELISA, which relies on specific reagents, mass spectrometry-based assays measure the molecular mass directly. This offers:

        • Faster development
        • Increased specificity
        • No reliance on recombinant antigens or anti-idiotypic antibodies

    Why Choose AxisPharm?

        • Advanced Technology: We use state-of-the-art mass spectrometry to deliver fast, precise results.
        • Comprehensive Analysis: Our services detect PTMs, protein heterogeneity, and sequence variations.
        • Biopharmaceutical Expertise: We specialize in mAb characterization and other complex protein studies.
        • Tailored Solutions: Whether working with antibodies or other proteins, we customize our services to your needs.

    Deliverables

        • A detailed report with:

          • Molecular weight determination
          • Detected PTMs
          • Insights on protein heterogeneity

        • An Excel or HTML file with:

          • Complete qualitative and quantitative data
          • Detailed analysis of modifications and structural insights

    Turnaround Time: 2-4 weeks

    Request an example report for more information.

    Contact Us

    AxisPharm provides top-tier intact protein analysis using MALDI-TOF, ESI-TOF, and LC-HRMS. Whether you’re working with monoclonal antibodies or other proteins, we deliver accurate, fast results. Contact us today for more details and technical support!

    Send Request

      Intact Protein Analysis Related Products:

      Fluorescent Dyes

      Related service:

      Bioconjugation Services

      Related Reading:

      Proteomics Service

      Protein Separation And Purification

      Protein Precipitation Technical Guide

      Plasma Protein Binding Assay