The plasma protein binding assay measures the proportion of compounds that are not bound (free) to the protein in the plasma by equilibrium dialysis. Since only the free compound can bind to its target and produce activity, and the compound bound to plasma protein may have a longer half-life and lower clearance rate, the determination of plasma protein binding rate is very important for understanding the activity of the compound and tissue distribution.
Plasma Protein Binding Assay Protocol（Equilibrium Dialysis）
| Method | Equilibrium Dialysis（100% Plasma） |
| Species | Rat, mouse, dog, monkey, human |
| Test concentration | 2 µM （DMSO final concentration <0.5%） |
| Positive Control | Warfarin（other compounds available） |
| Compound required | 100 µL 10mM DMSO solution or 2 mg |
| Analysis method | LC-MS/MS |
| Data Delivery | Fraction unbound in 100% Plasma Recovery |
Send Request
Other bioanalytical services in vitro ADME tests from Axispharm bioanalytical laboratory:
- Solubility Test
- Microsomal Stability Assay (Mouse, Rat, Dog, and Monkey)
- Hepatocytes Stability Assay
- CYP450 Inhibition Assay
- MDCK-MDR1 Permeability Assay
- Caco-2 Permeability Assay
- Red Blood Cell Partitioning
- LogD
Related Reading: