Cytochrome P450 Assay at AxisPharm Laboratories
Overview
AxisPharm Laboratories offers specialized Cytochrome P450 (CYP450) assays to assess how drugs are metabolized by the liver. Cytochrome P450 enzymes play a crucial role in drug metabolism, impacting how drugs are processed, their potential interactions, and their overall effectiveness. Our assays help in evaluating these factors, supporting drug development and safety assessments.
P450-CYP-Inhibition-Assay LCMSMS Optimization
Key Features
- Enzyme Activity Analysis: Measures how test compounds are metabolized by CYP450 enzymes. This helps determine how drugs are processed and their potential for interacting with other drugs.
- Drug-Drug Interaction Testing: Evaluates whether a compound inhibits or induces CYP450 enzymes, which is vital for predicting possible drug interactions.
- Pharmacokinetic Insights: Provides data on how drugs are metabolized, helping to predict their behavior in the body.
How It Works
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Enzyme Preparation:
- We use either recombinant CYP450 enzymes or liver microsomes enriched with these enzymes.
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Incubation:
- Test compounds are incubated with the CYP450 enzyme preparations in a controlled environment.
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Detection:
- Metabolites are analyzed using techniques such as high-performance liquid chromatography (HPLC) or liquid chromatography-mass spectrometry (LC-MS).
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Data Analysis:
- We assess enzyme activity based on the production of metabolites and calculate key parameters like Km (Michaelis constant) and Vmax (maximum reaction velocity).
Applications
- Drug Development: Understand how new drugs are metabolized to design better compounds.
- Safety Studies: Identify metabolic pathways that may lead to toxic effects.
- Regulatory Support: Provide critical data for regulatory submissions and drug approvals.
Why Choose AxisPharm?
- Expertise: Our team provides precise and reliable Cytochrome P450 assays.
- Advanced Technology: We use cutting-edge equipment for accurate measurements.
- Comprehensive Reporting: Detailed results and insights to aid in drug development decisions.
Available Enzymes
We offer assays for a range of CYP450 enzymes, including:
- CYP1A2
- CYP2D6
- CYP3A4
- CYP2C19
- CYP2C9
Contact Us
For more information or to schedule a Cytochrome P450 assay, please contact AxisPharm Laboratories.
Cytochrome P450 Test (CYP) Inhibition Assay Protocol (Single Point)
| Compound test concentration | 10µM，0µM |
| CYP450 isoforms | 1A2，3A4, 2C9, 2C19, 2D6 |
| Microsome concentration | 0.05-0.5mg/mL |
| Cofactor | NADPH |
| Incubation duration | 5-40min |
| IC50 calculation | |
| Data Interpretation | IC50<1 µM Strong Inhibitor 1µM IC50>10 µM Weak or Non Inhibitor |
Know our lab test cost, please go to https://axispharm.com/price-list/.
Ref:
Camilla Bernasconi, Olavi Pelkonen, Tommy B. Andersson, Judy Strickland, Iwona Wilk-Zasadna, David Asturiol, Thomas Cole, Roman Liska, Andrew Worth, Ursula Müller-Vieira, Lysiane Richert, Christophe Chesne, Sandra Coecke, Validation of in vitro methods for human cytochrome P450 enzyme induction: Outcome of a multi-laboratory study, Toxicology in Vitro, Volume 60, 2019, Pages 212-228, ISSN 0887-2333.
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