The liver is the most important organ for drug metabolism and elimination in the human body, so testing the stability of the compound in the liver cells is very important to understand the metabolism of the compound in the liver cells in the body. Unlike microsomal stability assay, liver cells contain all liver enzymes involved in drug metabolism (including phase I and phase II), so hepatocyte stability assay liver cell stability analysis can be used to study metabolites of phase I and phase II metabolism. In addition, comparing the stability of compounds in liver cells of different species helps to understand the differences in the metabolism of compounds in different species.

Protocol for Hepatocyte Stability Assay

CellsCryopreserved hepatocytes
SpeciesHuman, rat, mouse, dog, monkey
Test Compound Concentration3 µM
Incubation duration0, 5, 10, 20, 40, 60 min
Compounds required10 mM DMSO solution,50 μL
Analysis MethodLC-MS/MS
Control compoundUnknown substrates for phase I and phase II metabolism
Data deliveryIntrinsic clearance
Standard error of intrinsic clearance
Half life

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