Oligonucleotides generally refer to linear polynucleotide fragments consisting of 2 to 10 nucleotide residues linked by phosphodiester bonds, but when this term is used, the number of nucleotide residues is not strictly It is stipulated that in many literatures, polynucleotide molecules containing 30 or more nucleotide residues are also called oligonucleotides. Oligonucleotides can be synthesized automatically by instruments, and they can be used as primers for DNA synthesis, gene probes (Probe), etc., and are widely used in modern molecular biology research.
Oligonucleotides are easily linked to their complementary pairs.
Oligonucleotides are often used as probes to determine the structure of DNA or RNA, and are used in gene chips, electrophoresis, fluorescence in situ hybridization and other processes.
The DNA (deoxyribonucleic acid) synthesized by oligonucleotide can be used for chain polymerization reaction, which can amplify and determine almost all DNA fragments. A replica of DNA.
Regulatory oligonucleotides are used to inhibit RNA fragments and prevent their translation into proteins, which can play a certain role in inhibiting the activity of cancer cells.
Both Q-TOF-LC-MS and TQ-LC-MS can be used for mass determination and quantification analysis of therapeutic oligonucleotides.
QTOF-LC-MS is a high-performance liquid chromatography (LC) tandem mass spectrometer (LC-MS/MS) combined with a quadrupole time-of-flight mass spectrometer (Q-TOF). High resolution TOF mass spectrometer can be used for quality characterization, quality control and quantification in the analysis of oligonucleotides.
Quantitative analysis of oligonucleotides can also be achieved by using a high-performance liquid chromatography-triple quadrupole mass spectrometer (TQ-LC-MS). TQ-LC-MS is an LC-MS/MS combining LC and a triple quadrupole mass spectrometer (TQ). It is possible to carry out selective and supersensitive analysis by multi-reaction monitoring (MRM), and it can be utilized for analysis of pharmacokinetics etc. in the analysis of therapeutic oligonucleotides.
When the difference between the target oligonucleotide and the impurities in the therapeutic agent is the structural difference only due to a slight modification, it is assumed to be undistinguishable by LC-UV detection because the chromatographic separation is difficult. Mass spectrometer based analysis, such as Q-TOF-LC-MS and TQ-LC-MS , on the other hand, are considered to be capable of highly selective detection due to their high mass resolution, mass accuracy, selectivity and sensitivity, even when chromatographic separation is not possible.
AxisPharm offers both TQ-LC-MS and QTOF-LC-MS for oligonucleotide analysis. We are fully engaged in providing oligonucleotide analysis services to support COVID-19 research for cure.