Cassette PK (I.V.) study is a procedure for higher-throughput screening in drug discovery to rapidly assess pharmacokinetics of large numbers of candidate compounds. In this procedure, multiple compounds are administered simultaneously to a single animal. Blood samples are collected, and the resulting plasma samples obtained are analyzed by means of an assay method such as liquid chromatography coupled to tandem mass spectrometry (LCMS/MS) that permits concurrent assay of many compounds in a single sample. Consequently, the pharmacokinetics of multiple compounds can be assessed rapidly with a small number of experimental animals and with shortened assay times. 

In the process of compound PK screening, in order to save costs, molecules with similar physical and chemical properties can be combined to perform a Cassette PK experiment, usually 3-5 molecules can be tested in one experiment. The reference protocol of the combined PK experiment is as follows.

Cassette PK Protocol

SpeciesMouse, rat, dog, monkey, cat, rabbit, guinea pig
Dosing routeP.O., I.V., I.M.; I. P. etc
GroupsOne group,P.O.or I.V.;
DosageP.O.: 5-10 mpk,I.V.:1-2 mpk for each compound
Dosing frequencySingle dose
Number of animals3 per group
Time points5min, 15 min,30 min, 1 hr, 2 hr, 4 hr, 8 hr, 24 hr
Analysis methodLC-MS/MS
Data analysisWinnonlin
Turn Around Time5-10 business days

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