The Bruker Autoflex Max MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometer) is a powerful analytical tool designed for the comprehensive characterization of biopolymers such as proteins, protein digests, and oligonucleotides, as well as organometallic complexes, synthetic polymers, and other high molecular weight organic materials. This advanced instrument combines MALDI technology with a TOF mass analyzer, providing high-resolution, accurate mass spectrometry capabilities.

Bruker Autoflex Max

Key Features and Capabilities:

  1. MALDI Coupled with TOF Mass Analyzer: The instrument’s combination of MALDI with TOF mass analysis allows for the efficient and precise characterization of complex biomolecules and high molecular weight materials without fragmentation, preserving the integrity of the samples.
  2. SmartBeam-II™ Laser Technology: Proprietary to Bruker, this laser technology features up to a 2 kHz repetition rate, enabling rapid and efficient sample ionization with high resolution and sensitivity.
  3. High Dynamic Range FlashDetector™ System: Combined with a 10-bit 5 Gs/s digitizer, this system delivers unmatched dynamic range and superior data acquisition capabilities, ensuring high mass accuracy and resolution.
  4. PAN™ Wide Mass Range Focusing: This feature enhances the resolution power and mass accuracy across a wide mass range, making it suitable for a broad array of applications, from proteomics to material science.
  5. MALDI-Ion Source with Integrated Self-Cleaning: The instrument includes a MALDI ion source equipped with a self-cleaning procedure, reducing maintenance needs and ensuring consistent performance.
  6. TOF-Analyzer for Multiple Modes: The TOF analyzer supports Linear and Reflectron modes, MS/MS, and high-energy CID (collision-induced dissociation) capabilities, providing versatility for different analytical needs. It also operates in both positive and negative ion modes, expanding its applicability.
  7. High Resolution Performance: Achieves resolution performance of RP > 26,000 FWHM (Full Width at Half Maximum) in MS mode, delivering clear and precise mass spectra.
  8. Superb Low PPM Mass Accuracy: Offers low parts-per-million (ppm) mass accuracy for both MS and MS/MS modes with internal calibration, ensuring highly accurate identification and quantification of analytes.
  9. Fast Data Acquisition: Capable of up to 2 kHz data acquisition speed in both linear and reflectron modes, allowing for quick and efficient analysis of samples.

Applications:

  • Biopolymers: Ideal for analyzing proteins, protein digests, and nucleic acids, facilitating research in proteomics and genomics.
  • Synthetic Polymers and High Molecular Weight Organics: Used extensively in material science for analyzing polymers and complex organic materials.
  • Organometallic Complexes: Allows detailed analysis of complex compounds in organometallic chemistry.
  • MS/MS and High Energy CID: Supports structural elucidation of complex molecules through tandem mass spectrometry and high-energy collision-induced dissociation.

The Bruker Autoflex Max MALDI-TOF MS stands out for its advanced technology, high-speed data acquisition, and superior accuracy, making it an invaluable tool in both research and industrial settings for the analysis of a wide range of complex materials.