Protein sequencing and quantification – TMT

Protein Sequencing and Quantification with TMT Labeling

At AxisPharm, we provide expert protein sequencing and quantification using TMT (Tandem Mass Tag) labeling. This service is ideal for both quantitative and qualitative analysis of biological systems. Whether you need to identify proteins or measure changes in protein levels across multiple samples, our advanced mass spectrometry technology ensures fast and reliable results.

TMT Meaning: What Does TMT Mean?

TMT (Tandem Mass Tag) is a method used in proteomics to label peptides. It allows you to compare protein levels across different samples in a single mass spectrometry experiment. This makes TMT proteomics a powerful tool for accurate and efficient protein analysis.

How Does TMT Labeling Work?

TMT labeling tags peptides from each sample with unique labels. These tags have the same mass, so they behave identically in mass spectrometry. During the MS/MS stage, the tags are broken down into reporter ions. These ions are unique to each sample, allowing us to easily quantify proteins from each condition.

TMT Plex Options

We offer a variety of TMT plex options to meet your research needs. These options allow you to analyze multiple samples in one run:

• 4 plex
• 6 plex
• 8 plex
• 10 plex
• 16 plex
• 18 plex

These choices give you high-throughput analysis for efficient TMT proteomics.

For more comprehensive services, we also provide cell culture, cell treatment, and full proteomics analysis. Feel free to contact us for more information.

Steps in TMT Protein Quantification

1. Sample Preparation:

   • We start with protein extraction and trypsin digestion. Afterward, we apply TMT labeling to tag peptides from your samples. We then pool the labeled peptides and fractionate them to ensure broad protein coverage.

2. LC-MS/MS Analysis:

   • We use the ThermoFisher Fusion Lumos mass spectrometer to analyze your samples. This step provides high-quality data for protein identification and quantification.

3. Data Analysis:

   • We identify and quantify proteins using the mass spectrometry data. Next, we perform differential analysis to see how protein levels change across your samples.

4. Report Generation:

   • You will receive a detailed report in PDF, Excel, or HTML format. The report includes a list of identified proteins, testing methods, and key findings from the analysis.

Deliverables

• A comprehensive report with:
  • Sample details
  • Testing methods used
  • A summary of the data and key results
• An Excel workbook or HTML page with:
  • Detailed qualitative and quantitative protein identification
  • Statistical analysis of protein level changes

Turnaround Time: 1-3 weeks

An example report is available upon request.

---

Why Choose AxisPharm?

AxisPharm offers TMT proteomics with advanced mass spectrometry to deliver fast and precise protein analysis. Our range of TMT plex options and detailed reporting make us the perfect choice for your proteomics needs. Whether you’re studying protein expression, discovering biomarkers, or running large-scale experiments, we provide the data you need—quickly and reliably.

Contact us today to get started with your TMT labeling project! An example report is available upon request.