In a single-dose PK experiment, the bioavailability of small-molecule drugs can be calculated by comparing the drug exposure of oral administration and systemic administration through a single administration and then testing the blood drug concentration of the compound in the animal.
Single-dose PK Study Protocol
| Species | Mouse, rat, dog, monkey, cat, rabbit, guinea pig |
| Dosing route | P.O., I.V., I.M.; I. P. etc |
| Groups | 2 groups, one for P.O. dosing, one for I.V. dosing |
| Dosage | P.O.: usually 5-10 mpk I.V.：usually 1-2 mpk |
| Dosing frequency | Single dose |
| Number of animals | 3 per group, 6 in total |
| Time points | 5min, 15 min，30 min, 1 hr, 2 hr, 4 hr, 8 hr, 24 hr （other time points can be chosen） |
| Compound required | 10-20 mg (if formulation test is needed, more compound is required) |
| Analysis method | LC-MS/MS, standard curve |
| Data analysis | WinNonlin |
| Turn Around Time | 5-10 business days |
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