Blood Plasma Ratio (BP) is one of the important parameters to measure the distribution of drugs from blood to tissues and organs, that is, the concentration ratio of compounds in whole blood and plasma, respectively.
The blood used in the experiment comes from humans, rats, mice, dogs, monkeys, etc. The experimental process is to add the drug to the whole blood and the plasma obtained by centrifugation, respectively, in a water bath at 37 °C, centrifuge the whole blood after 1 h, and take out the whole blood supernatant and plasma, through LC-MS detection, calculate the compound in the whole blood. , the distribution ratio in plasma.
It should be noted in the experiment that the amount of whole blood used and the amount of plasma obtained after centrifugation need to be accurately quantified, because the size of BP and drug properties are related to hematocrit (H). Hematocrit refers to the volume percentage of blood cells in whole blood, so accurate quantification of whole blood and plasma is a key step in the experiment.
Compounds have different distribution behaviors in the blood of different species and different individuals of the same species. Determining the whole blood/plasma concentration ratio of a drug can help to better understand the pharmacokinetic properties of the drug, and can also evaluate the potential blood toxicity. for reference.
Axispharm Blood to Plasma Ratio Assay Protocol
| Species | Rat, mouse, dog, monkey, human |
| Test concentration | 0.5 µM （other concentrations can be used) |
| Positive Control | Methazolamide (human) Chlorthalidone (rat and mouse) Chloroquine (dog) |
| Compound required | 100 µL 10mM DMSO solution or 2 mg |
| Replicates | N=3 |
| Analysis method | LC-MS/MS |
| Data Delivery | Mean±SEM of blood to plasma ratio |