Untargeted Metabolomics Services: Comprehensive Metabolite Profiling

At AxisPharm, we offer untargeted metabolomics services for broad, unbiased analysis of metabolites. Using advanced mass spectrometry and chromatography, we ensure thorough profiling and quantification of the metabolome. As a result, researchers gain crucial insights across various fields, including cerebrospinal fluid (CSF) metabolomics.

What is Untargeted Metabolomics?

Untargeted metabolomics analyzes a wide range of metabolites in biological samples without requiring prior knowledge of which ones are present. Therefore, this method captures both known and unknown metabolites, providing a complete picture of the metabolome. In contrast, metabolomic fingerprinting focuses on identifying patterns but does not quantify specific metabolites.

Untargeted-Metabolomics-Analysis-of-4-MeO-α-PVP

Untargeted-Metabolomics-Analysis-of-4-MeO-α-PVP

Why Choose Untargeted Metabolomics?

  1. Comprehensive Profiling: You can identify and quantify a wide range of metabolites in just one run.
  2. Biomarker Discovery: Through this approach, you can uncover new biomarkers related to diseases, drug responses, and metabolic disorders, including via CSF metabolomics.
  3. Systematic Analysis: By understanding metabolic pathways and biological changes, you can drive new discoveries.
  4. Data-Driven Insights: With rich and detailed data, you can generate hypotheses and make significant advances in your research.

Our Untargeted Metabolomics Services

1. Sample Preparation and Extraction
We handle a variety of biological samples such as blood, urine, tissues, and cerebrospinal fluid (CSF). By using techniques like liquid-liquid extraction, solid-phase extraction, and protein precipitation, we ensure efficient sample preparation for accurate results.

2. Advanced Analytical Techniques

  • Mass Spectrometry (MS): High-resolution MS ensures precise identification and quantification of metabolites.
  • Liquid Chromatography-Mass Spectrometry (LC-MS): Combining chromatography with MS allows for effective analysis of complex metabolite mixtures.
  • Gas Chromatography-Mass Spectrometry (GC-MS): This technique is especially useful for volatile and semi-volatile metabolites, providing accurate results.

3. Data Analysis and Interpretation

  • Metabolite Identification: We use comprehensive databases to accurately identify both known and unknown metabolites.
  • Quantification: Our advanced algorithms provide precise quantification of the full range of the metabolome.
  • Pathway Analysis: Additionally, we integrate your data with pathway databases to detect key metabolic shifts.
  • Statistical Analysis: Through advanced statistical tools, we can reveal patterns and discover potential biomarkers.

Metabolomic Fingerprinting vs. Profiling

  • Metabolomic fingerprinting identifies patterns within the metabolome, which is useful for classification or diagnosis, but it doesn’t quantify individual metabolites.
  • Metabolomic profiling goes further by identifying and quantifying specific metabolites, offering deeper insights into their biological roles.

Applications of Untargeted Metabolomics

  • Biomedical Research: You can study how diseases like cancer, diabetes, and cardiovascular conditions affect the metabolome.
  • Pharmaceutical Development: This service helps you assess drug effects on metabolism, ensuring both safety and efficacy.
  • Nutritional Science: You can explore how diets and supplements influence metabolic processes and overall health.
  • Environmental Impact: Analyze how environmental factors affect the metabolome, giving you a better understanding of external influences on health.

Why Choose AxisPharm?

  1. Expertise and Technology
    • We use state-of-the-art instruments such as LC-MS and GC-MS to ensure precise and accurate analysis.
    • Our PhD-level scientists bring extensive expertise in metabolomics and data interpretation, which guarantees high-quality results.
    • Moreover, we enforce strict quality control throughout the process to deliver reliable data.
  2. Comprehensive Services
    • We customize our metabolomics services to fit your specific research goals, ensuring that our work aligns with your objectives.
    • Our team offers guidance from study design to final data interpretation, providing support at every step.
    • Additionally, we handle every aspect of the process, from sample preparation to comprehensive reporting, for a smooth experience.

Our Process

  1. Consultation: First, we collaborate with you to design a custom metabolomics study based on your research needs.
  2. Sample Preparation: Next, we extract metabolites from your biological samples using advanced preparation techniques to ensure quality.
  3. Analysis: Our experts then conduct a thorough analysis using mass spectrometry and chromatography, generating precise data.
  4. Data Processing: After analysis, we interpret your data using advanced tools and databases, providing you with clear, actionable insights.
  5. Reporting: Finally, we deliver a detailed report that includes insights, visualizations, and recommendations to help you move forward with your research.

Get Started with Untargeted Metabolomics

Now is the time to advance your research with AxisPharm’s untargeted metabolomics services. Contact us today to learn how our comprehensive metabolite profiling can give you the insights you need to take your projects to the next level.

AxisPharm—Your Partner in Comprehensive Metabolomics Analysis.

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    Ref:

    Wu, Hsin-Yi; Chen, Yuan-Chih; Hsu, Jing-Fang; Lu, Hsiang-Ting; Pan, Yu-Yi; Ma, Mi-Chia; and Liao, Pao-Chi (2023) “Untargeted metabolomics analysis assisted by signal selection for comprehensively identifying metabolites of new psychoactive substances: 4-MeO-α-PVP as an example,” Journal of Food and Drug Analysis: Vol. 31 : Iss. 1 , Article 9.