Post Translational Modification Analysis

At AxisPharm, we offer expert Post Translational Modification (PTM) analysis to help you understand how proteins are modified after they are made. PTMs are essential for regulating protein function, stability, and interactions. Our advanced mass spectrometry technology detects and quantifies these modifications, giving you clear insights into how proteins behave in biological systems.

Orbitrap Fusion Lumos Tribrid Mass Spectrometer for Post Translational Modification (PTM) Analysis

What Does PTM Mean?

Post-translational modifications (PTMs) are chemical changes proteins undergo after translation. These changes, such as phosphorylation, acetylation, glycosylation, and ubiquitination, play a major role in:

• Regulating protein activity
• Controlling cellular signaling
• Driving protein interactions
• Influencing disease development

Specific proteins, like SFPQ (Splicing Factor Proline and Glutamine Rich), are known to have unique post-translational modifications that impact gene expression and cell function. Analyzing these modifications is key to understanding disease mechanisms and developing new treatments.

Post-Translational Processing and PTM Analysis

Post-translational processing, including various PTMs, directly affects how proteins function. Our PTM analysis service offers both quantitative and qualitative insights into these modifications, helping you explore how they impact protein roles in the body.

Key Features of Our PTM Analysis

1. Comprehensive PTM Identification:
   We analyze a wide range of PTMs, including phosphorylation, acetylation, ubiquitination, and more.

2. Advanced Mass Spectrometry:
   Our mass spectrometry technology ensures precise and accurate detection of PTMs.

3. Quantitative and Qualitative Data:
   We provide both quantitative data on modification levels and qualitative data on the types of PTMs present.

4. Precise Site Mapping:

   We identify the exact sites where PTMs occur, helping you understand their specific effects on protein function.

How We Perform PTM Analysis

1. Sample Preparation:
   We start by extracting proteins from your sample and digesting them into peptides.

2. Enrichment (when needed):
   For some modifications like phosphorylation, we enrich the modified peptides to ensure better detection.

3. LC-MS/MS Analysis:
   Our mass spectrometer identifies and quantifies the PTMs, providing detailed information on each modification.

4. Data Analysis:
   We analyze the data to identify the types of modifications, their locations, and any changes in modification levels across your samples.

5. Report Generation:
   We create a comprehensive report that includes the identified PTMs, their locations, and key findings from the analysis.

Deliverables

• A report that details:
  • Types of post-translational modifications
  • PTM locations and frequency
  • Sample and method details
  • A summary of quantitative PTM data
• An Excel file or HTML report with:
  • Complete qualitative and quantitative PTM data
  • Statistical analysis of modification changes across samples

Why Choose AxisPharm for PTM Analysis?

• Advanced Technology: Our mass spectrometry provides accurate, high-quality results for all types of PTMs.
• Wide Range of PTMs: We cover everything from SFPQ post-translational modifications to common PTMs like phosphorylation and glycosylation.
• Tailored Solutions: We customize our analysis to fit your specific research needs, whether for basic science or drug discovery.

Contact us today to learn more about how our post-translational modification analysis can support your research! An example report is available upon request.