Metabolic Flux Analysis (MFA) with AxisPharm

What is Metabolic Flux Analysis (MFA)?

Metabolic Flux Analysis (MFA) measures how quickly metabolic reactions happen within cells. This technique helps us better understand cellular processes, energy use, and biosynthesis. MFA is especially important in biotechnology and drug development, where understanding how cells process nutrients and drugs is crucial for developing treatments and optimizing production methods.

C13 Isotope Tracing at AxisPharm

AxisPharm uses C13 isotope tracing with tracers like 13C glucose and 13C palmitate. By following these labeled molecules through cell pathways, researchers can identify important routes and potential drug targets. This approach gives clear insights into cell functions and shows how cells react to different treatments.

Key Applications

  • Biotechnology Pathway Optimization
    C13 tracing shows nutrient processing in cells, improving production pathways and boosting efficiency and yield.

  • Drug Development and Toxicity Testing
    C13 MFA reveals drug effects on cell metabolism, aiding in toxicity identification and enhancing drug design and safety.

  • Cell Survival and Apoptosis
    C13 tracing identifies pathways linked to cell survival and death, supporting disease research and therapy development.

  • Discovering New Pathways
    C13 labeling uncovers unknown pathways, offering fresh insights into cell functions and disease progression.

MFA Techniques Used at AxisPharm

  • Stable Isotope Labeling with C13
    Trace nutrient and energy flows in cells.

  • Computational Modeling
    Interpret C13 data to show which pathways cells prioritize.

  • Flux Balance Analysis (FBA)
    Predicts optimal metabolic states and confirms the actual pathways in use.

Benefits of C13 MFA with AxisPharm

  • Precise Pathway Mapping: By using C13 tracing, we gain a clear view of which metabolic pathways are active in cells.
  • Real-Time Data: C13 labeling provides live insights into cellular metabolism, which is essential for timely data on metabolic changes.
  • Versatile Applications: C13 MFA is valuable across various fields, from biotechnology to drug research, supporting a wide range of research and industry goals.

Conclusion

AxisPharm’s C13 Metabolic Flux Analysis enables researchers to accurately map cell metabolism, identify essential pathways, and discover potential drug targets. By using C13 MFA, AxisPharm drives advancements in understanding cellular functions, disease mechanisms, and therapeutic development.