AxisPharm offers a range of high-purity Tetrazine PEG reagents with different linker lengths. Different kinds of Tetrazine PEG linkers may be available by custom synthesis. Fast shipping.
The tetrazine ligation is the reaction of a trans-cyclooctene and an s-tetrazine in an inverse electron demand Diels Alder (IEDDA) reaction followed by a retro-Diels Alder reaction to eliminate nitrogen gas. This is a fast reaction with a second-order rate constant of 2000 M−1-s−1 (in 9:1 methanol/water) allowing modification of biomolecules at very low concentrations. This ligation can be performed in aqueous media at a very fast speed. Reactions have also been performed using TCO or norbornenes as dienophiles at second-order rates on the order of 1 M−1–s−1 in aqueous media. The reaction has been applied in labeling live cells and other bio-entities.